The Scientific research center offer the following techniques provided with a short brief and their applications: -

1-Gas chromatography (GC)

Gas chromatography is an instrumental method for the separation and identification of volatile and thermally stable chemical compounds. The process is based on separating mixture of compounds into its individual components.

Clarus 580 Perkinelmer coupled with FID/TCD detectors. The GC system is easy to operate and deliver high performance, capacity, accuracy and throughput analytical lab demands. And Head space TurboMatrix sample handling technology giving most versatile portfolio of technologies that delivers unparalleled precision and provide exceptional flexibility for the most efficient sample preparation.

Applications of GC:-

Various chemical compounds are analyzed by GC in different fields such as Agricultural, medical, pharmaceutical, petrochemical, forensic, and biological analysis.

2-Gas Chromatography–Mass Spectrometry (GC-MS)

Gas Chromatography–Mass Spectrometry (GC-MS) is a hyphenated analytical technique that combines the separation properties of gas-liquid chromatography with the detection feature of mass spectrometry to identify different substances within a test sample. GC is used to separate the volatile and thermally stable substitutes in a sample whereas GC-MS fragments the analyte to be identified on the basis of its mass.

Clarus 580 Gas chromatograph coupled with Clarus SQ 8S mass spectrometer offers unsurpassed sensitivity and unparalleled stability for identification and quantification of volatile and semi-volatile compounds. It delivers high thouhgputs, accuracy, is rugged and dependable, and provide reliable results.

Applications of GC/MS:-

Drug detection, Fire investigation, Cosmetic, forensic, petrochemical, Food and Environmental analysis, Explosives investigation and identification of unknown samples.

3-High performance-Liquid chromatography (HPLC):

It's the most widely used techniques that involves the separation of liquid mixture sample into its individual parts by passing through stationary phase with carrier liquid mobile phase delivered by a pump. These components are separated from one another by the column packing that involves various chemical and/or physical interactions between the molecules and the packing particles.

Flexar perkinelmer HPLC offers the right technology for testing samples both efficiently and accurately. The system is easy to operate, relaiable, and ideal for routine analysis.

Applications of HPLC:-

Clinical diagnosis of diseases, disorders.  In scientific research for discovery. In pharmaceutical labs for analysis. In food industry for quality control. For standards control by government. For separation of similar molecules.

4-Atomic absorption spectroscopy (AAS):

Is an analytical technique that measures the concentrations of elements. It makes use of the wavelengths of light specifically absorbed by an element. The atoms absorb ultraviolet or visible light That correspond to the energies needed to promote electrons from one energy level to another higher energy level.

PinAAcle 900T perkinelmer AAS has the flexibility to switch between flame and furnace in seconds, and its high light throughput optical system, combined with a solid-state detector, provides the highest-quality efficiency and signal-to-noise performance of any AA system on the market. In addition, it features cutting-edge fiber optics for improved detection limits and TubeView™ color furnace camera for easier autosampler tip alignment and sample dispensing, as well as to monitor drying and pyrolysis during analysis for simpler method development. All of this in the smallest flame/furnace AA footprint, saving valuable bench space.

Applcations of AAS:-

Determination of metal elements in blood, saliva, urine samples like sodium, potassium, magnesium, calcium, and heavy metals like iron, manganese, copper, zinc, mercury, lead, nickel, tin, etc. in urine, blood, etc., and estimation of Lead in petroleum products. and also, for determination of metal concentrations in groundwater and bore well samplings before using for drinking and irrigation.

5-Liquid chromatography-Mass spectrometry (LC-MS):-

It's an analytical technique that combines the physical separation capabilities of liquid chromatography (or HPLC) with the mass analysis capabilities of mass spectrometry (MS). The sample is separated by LC, and the separated sample species are sprayed into atmospheric pressure ion source, where they are converted into ions in the gas phase. The mass analyzer is then used to sort ions according to their mass to charge ratio and detector counts the ions emerging from the mass analyzer and may also amplify the signal generated from each ion. As a result, mass spectrum is created, which is used to determine the elemental or isotopic nature of a sample, the masses of particles and of molecules, and to elucidate the chemical structures of molecules.

Flexar Perkinelmer LC/MS deliver the speed, sensitivity, exact mass information, wide dynamic range, and mass accuracy needed for having complete confidence in results. LC/MS solutions are designed to provide rapid and accurate mass identification and quantification.

Applications of LC/MS:-

Identification and quantification of chemical species in food, biomedical, environmental, pharmaceutical fields. And also it has various applications in Biochemical Screening for Genetic Disorders, Therapeutic Drug Monitoring and Toxicology, Steroid Hormones, Vitamins and related metabolites.

6-CHNS/O Elemental Analyzer:

It’s a technique that determines the percentage of carbon, hydrogen, nitrogen, sulphur and oxygen of organic compounds based on the principle of ''Dumas method'' which involves the complete and instantaneous oxidation of the sample by ''flash combustion''. The combustion products are separated by a chromatographic column and detected by thermal conductivity detector, which gives an output signal proportional to the concentration of the individual components of the mixture.

The 2400 Series II CHNS/O Elemental Analyzer is easy-to-use and capable of operating in three modes: CHN, CHNS and Oxygen, is fully automated, and includes a 60 position autosampler. It can handle a wide variety of sample types including solids, liquids, volatile and viscous samples.

Applications of CHNS/O:-

·         Pharmaceutical / Chemical: confirmatory analysis, purity

·         Energy: C/H Ratio of fuel, BTU analysis of coal, impurities analysis

·         Polymers: blend analysis, nitrogen level

·         Environmental: C/N ratio in soil, sediment and plant material, organic particulate collected on filters, nitrogen content in fertilizer

·         Food / Animal Feed: protein content, product labeling

Explosives: nitrogen content